Change in hydrolytic enzyme efficiency over time

Change in hydrolytic enzyme efficiency over time.The purpose of this study was to determine the action of hydrolytic enzymes (by Dyadic Cellulase CP CONC, and theDyadic Xylanase 2 XP CONC)over time. Chromatographicanalysis of holocellulose samples subjected to enzymatic hydrolysis was performed. The following hydrolysis parameters were used: time 48h, temperature 45⁰C, acetate buffer pH 5.4, commercial enzymes Dyadic.Holocellulose extracted by the sodium chlorite method from white poplar wood (PopulusalbaL.) was used. The final yieldof enzymatic hydrolysis was determined. The results of hydrolysis performed at intervals were compared.The results obtained show that the hydrolysis yield of holocellulose after five months decreased by 40 p.p. for glucose yield and by 25 p.p. for xylose yield. The yield for glucose after two and a half years decreases by 68 p.p. and 62 p.p. for xylose compared to the initial yield.